Human VB6 (Vitamin B6) ELISA Kit
Product Specifications
Product Name Alternative
Pyridoxine
Reactivity
Human
Field of Research
Nutrition metabolism; Hormone metabolism
Assay Type
Competitive Inhibition
Assay Principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human VB6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VB6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VB6 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
2h
Standard
300 ng/mL
Sample Type
Serum, plasma and other biological fluids
Detection Range
4.69-300 ng/mL
Sensitivity
1.41 ng/mL
Available Sizes
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