Anti-DAP Kinase 2 Antibody

Rabbit Polyclonal Antibody specific to DAPK2

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

DAP kinase 2, EC 2.7.11.1, DAP-kinase-related protein 1, DRP-1

Gene Name

DAPK2

Gene ID

23604

NCBI Gene ID

UniProt

Q9UIK4

Accession Number

NP_055141.2

Cellular Locus

Cytoplasm. Cytoplasmic vesicle, autophagosome lumen.

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Peptide corresponding to aa 356-370 of human DAPK2 (accession no. BAA88063). This sequence is identical to that of mouse.

Target Antigen

Death-associated protein kinase 2

Target

DAP Kinase 2

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, IHC-P

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell death signals, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Acts as a mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation (PubMed:17347302). Regulates granulocytic motility by controlling cell spreading and polarization (PubMed:24163421). {PubMed:17347302, PubMed:24163421, PubMed:26047703}.; Isoform 2 is not regulated by calmodulin. It can phosphorylate MYL9. It can induce membrane blebbing and autophagic cell death.

Additionnal Information

Immunoblotting: use at 1ug/mL. Immunohistochemistry: use at 2ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: Whole cell lysate from A431cells.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human, mouse, and rat DAPK2 (approx. 42kDa) and does not cross- react with DAPK.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Certain serine/threonine protein kinases, such as RIP and DAP kinase, are mediators of apoptosis. DAP kinase (DAPK) is a pro- apoptotic, calcium-regulated serine/threonine kinase containing a death domain. Expression of DAPK induces cell death and suppresses oncogenic trans-formation. DAPK mediates IFN -induced apoptosis. A DAPK-related protein was recently described and designated DAPK2, or DRP-1. Expression of DAPK2 induces apoptosis. The messenger RNA for DAPK2 is expressed in multiple human tissues.