Furin Activity Assay Kit (Fluorometric)

Furin is a serine protease that belongs to the Proprotein Convertase Subtilisin (PCs) family. PCs family members are calcium-dependent serine endoproteases that process latent, immature precursor proteins into their biologically active, functional form. Furin is predominantly localized in the Golgi apparatus, where it functions to cleave other proteins into their mature, active forms. However, it cycles between Golgi, cell surface, and the endosomes. Furin plays an important role in numerous processes including cell survival, migration, maintenance of homeostasis, embryogenesis, and in diseases. In addition to processing precursor proteins, Furin is utilized by numerous viral and bacterial pathogens for enhancing their virulence and spread. For example, the envelope protein of viruses such as HIV, influenza, several filoviruses including Ebola and Marburg virus, and the spike protein of SARS-CoV-2 must be cleaved by furin or furin-like proteases to become fully functional. Relatively little is known about the activity of Furin in humans. Understanding the activity levels of Furin in human biology could lead to a better understanding of its interaction with other PCs, trafficking itinerary and its role in human diseases. BioVision’s Furin Activity Assay Kit provides a quick, easy and sensitive method to determine Furin activity in various samples. In this assay, Furin activity is measured in the presence or absence of Furin Inhibitors. The difference between the fluorescence obtained in the presence or absence of Furin Inhibitors represents the actual Furin activity. A potent, specific Furin inhibitor is also included in the kit.