Recombinant Human Protein delta homolog 1 (DLK1), partial (Active)

Product Specifications

Product Name Alternative

Protein delta homolog 1; DLK-1; pG2; DLK1; DLK

Abbreviation

Recombinant Human DLK1 protein, partial (Active)

Gene Name

DLK1

UniProt

P80370

Expression Region

24-303aa

Organism

Homo sapiens (Human)

Target Sequence

AECFPACNPQNGFCEDDNVCRCQPGWQGPLCDQCVTSPGCLHGLCGEPGQCICTDGWDGELCDRDVRACSSAPCANNRTCVSLDDGLYECSCAPGYSGKDCQKKDGPCVINGSPCQHGGTCVDDEGRASHASCLCPPGFSGNFCEIVANSCTPNPCENDGVCTDIGGDFRCRCPAGFIDKTCSRPVTNCASSPCQNGGTCLQHTQVSYECLCKPEFTGLTCVKKRALSPQQVTRLPSGYGLAYRLTPGVHELPVQQPEHRILKVSMKELNKKTPLLTEGQ

Tag

C-terminal 10xHis-tagged

Type

Active Protein & In Stock Protein

Source

Mammalian cell

Field of Research

Signal Transduction

Relevance

May have a role in neuroendocrine differentiation.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Purity

Greater than 95% as determined by SDS-PAGE.

Activity

Yes

Bioactivity

①Measured by its binding ability in a functional ELISA. Immobilized Human DLK1 at 2 μg/mL can bind Anti-DLK1 recombinant antibody (CSB-RA006945MA1HU) . The EC50 is 1.598-1.804 ng/mL. ②Measured by its binding ability in a functional ELISA. Immobilized Human DLK1 at 2 μg/mL can bind Anti-DLK1 recombinant antibody (CSB-RA006945MA2HU) . The EC50 is 1.935-2.143 ng/mL.

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

31.2 kDa

References & Citations

Human urinary glycoproteomics; attachment site specific analysis of N- and O-linked glycosylations by CID and ECD. Halim A., Nilsson J., Ruetschi U., Hesse C., Larson G. Mol. Cell. Proteomics 11:1-17 (2012)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.