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Recombinant Streptococcus mutans serotype c UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-lysine ligase (murE)

Product Specifications

Product Name Alternative

L-lysine-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:L-Lys ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Abbreviation

Recombinant Streptococcus mutans serotype c murE protein

Gene Name

MurE

UniProt

Q8DST2

Expression Region

1-483aa

Organism

Streptococcus mutans serotype c (strain ATCC 700610 / UA159)

Target Sequence

MITIETVLTILKNNANFREIIDGKHYQYKYSNPEVAFHHISYDSRDIKASTLFFVKGATFKKEFLEKAIESGLTFYVAEKDYQVGIPLILVNNIKNAMCLIAKEFYDNPQDKLKTLAFTGTKGKTTAAYFAYHILKQGYRPAMISTMNTTLDGKTFFKSKLTTPESLDLFKMMAQAVANGMTHLIMEVSSQAYLVGRVYGLTFDVGVFLNISPDHIGPIEHPTFEDYFYHKRLLLKHSRYVIVNSQMNHFALIKEQVADQPHDFYGKYSDNQIINSKAFSFDLTGKLAGHYDIQLTGSFNQENAVAAALACLQLGASQTDIQKGIVQTTVPGRMEVLIQKNGAKVFVDYAHNGDSLEKLLSVVEEHQKGTLILILGAPGNKGESRRADFGYVINAHPELQVILTADDPNNEDPQLISQEIAHHIKRPVNIIVDRKQAIQKAMSLTQSENDAVIIAGKGADAFQIIKGKRTAYAGDIEVARKYL

Tag

C-terminal 6xHis-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Others

Relevance

Catalyzes the addition of L-lysine to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

60.9 kDa

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Protein Length

Full Length

Available Sizes

Frequently Asked Questions

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