Recombinant Streptococcus mutans serotype c UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-lysine ligase (murE)
Product Specifications
Product Name Alternative
L-lysine-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:L-Lys ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase
Abbreviation
Recombinant Streptococcus mutans serotype c murE protein
Gene Name
MurE
UniProt
Q8DST2
Expression Region
1-483aa
Organism
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Target Sequence
MITIETVLTILKNNANFREIIDGKHYQYKYSNPEVAFHHISYDSRDIKASTLFFVKGATFKKEFLEKAIESGLTFYVAEKDYQVGIPLILVNNIKNAMCLIAKEFYDNPQDKLKTLAFTGTKGKTTAAYFAYHILKQGYRPAMISTMNTTLDGKTFFKSKLTTPESLDLFKMMAQAVANGMTHLIMEVSSQAYLVGRVYGLTFDVGVFLNISPDHIGPIEHPTFEDYFYHKRLLLKHSRYVIVNSQMNHFALIKEQVADQPHDFYGKYSDNQIINSKAFSFDLTGKLAGHYDIQLTGSFNQENAVAAALACLQLGASQTDIQKGIVQTTVPGRMEVLIQKNGAKVFVDYAHNGDSLEKLLSVVEEHQKGTLILILGAPGNKGESRRADFGYVINAHPELQVILTADDPNNEDPQLISQEIAHHIKRPVNIIVDRKQAIQKAMSLTQSENDAVIIAGKGADAFQIIKGKRTAYAGDIEVARKYL
Tag
C-terminal 6xHis-tagged
Type
Developed Protein
Source
E.coli
Field of Research
Others
Relevance
Catalyzes the addition of L-lysine to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan
Endotoxin
Not test
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
60.9 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length
Available Sizes
Frequently Asked Questions
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