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Recombinant Mouse Acyl-coenzyme A thioesterase 2, mitochondrial (Acot2)

Product Specifications

Product Name Alternative

Acyl coenzyme A thioester hydrolase; MTE-I; Very-long-chain acyl-CoA thioesterase

Abbreviation

Recombinant Mouse Acot2 protein

Gene Name

Acot2

UniProt

Q9QYR9

Expression Region

43-453aa

Organism

Mus musculus (Mouse)

Target Sequence

NATLSVEPEGRSCWDEPLSIAVRGLAPEQPVTLRSALRDEKGALFRAHARYRADAGGELNLARAPALGGSFSGLEPMGLLWAMEPERPLWRLIKRDVQTPFLVELEVLDGHEPDGGQRLAQAVHERHFLAPGVRRVPVREGRVRATLFLPPEPGPFPGIIDLFGVGGGLLEYRASLLAGKGFAVMALAYYNYDDLPKSIETMHMEYFEEAVNYLRSHPEVKGPGIGLLGISKGGELGLAMASFLKGITAAVVINGSVAAVGNTISYKDETIPPVSLLRNQVKMTKDGLLDVVEALQSPLVDKKSFIPVERSDTTFLFLVGQDDHNWKSEFYADEISKRLQAHGKEKPQIICYPAAGHYIEPPYFPLCSAGMHLLVGANITFGGEPRAHAVAQVDAWQQLQTFFHKQLGSKS

Tag

C-terminal 6xHis-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Cardiovascular

Relevance

Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels. Displays higher activity toward long chain acyl CoAs (C14-C20) . The enzyme is involved in enhancing the hepatic fatty acid oxidation in mitochondria

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

51.9 kDa

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Protein Length

Full Length of Mature Protein

Available Sizes

Frequently Asked Questions

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