Recombinant Plutella xylostella NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9, partial
Product Specifications
Product Name Alternative
Complex I-B22; NADH-ubiquinone oxidoreductase B22 subunit
Abbreviation
Recombinant Plutella xylostella PLXY2_LOCUS10328 protein, partial
Gene Name
PLXY2_LOCUS10328
UniProt
A0A8S4FDC6
Expression Region
21-165aa
Organism
Plutella xylostella (Diamondback moth) (Plutella maculipennis)
Target Sequence
MAQVPVYGLASHARKVCSLYKKALRNLEMYYDRRHVYRYQAVLMRERFDQNAKIRDMRKATELLKAGEEELFLIQHPIPKKFTYSPGGVAFEREVEPPDWVLDYWHPLEKAEYPEYFKRREERKKQFIEMWEKEYGKEDPKSAHH
Tag
C-terminal 6xHis-tagged
Type
Developed Protein
Source
Baculovirus
Field of Research
Others
Relevance
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Endotoxin
Not test
Purity
Greater than 95% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
20.4 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Partial
Available Sizes
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