Recombinant Plutella xylostella NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9, partial

Product Specifications

Product Name Alternative

Complex I-B22; NADH-ubiquinone oxidoreductase B22 subunit

Abbreviation

Recombinant Plutella xylostella PLXY2_LOCUS10328 protein, partial

Gene Name

PLXY2_LOCUS10328

UniProt

A0A8S4FDC6

Expression Region

21-165aa

Organism

Plutella xylostella (Diamondback moth) (Plutella maculipennis)

Target Sequence

MAQVPVYGLASHARKVCSLYKKALRNLEMYYDRRHVYRYQAVLMRERFDQNAKIRDMRKATELLKAGEEELFLIQHPIPKKFTYSPGGVAFEREVEPPDWVLDYWHPLEKAEYPEYFKRREERKKQFIEMWEKEYGKEDPKSAHH

Tag

C-terminal 6xHis-tagged

Type

Developed Protein

Source

Baculovirus

Field of Research

Others

Relevance

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Endotoxin

Not test

Purity

Greater than 95% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

20.4 kDa

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.