Recombinant Human Claudin-9 (CLDN9), partial-Detergent (Active)

Product Specifications

Product Name Alternative

Claudin-9; CLDN9

Abbreviation

Recombinant Human CLDN9 protein, partial-Detergent (Active)

Gene Name

CLDN9

UniProt

O95484

Expression Region

2-217aa

Organism

Homo sapiens (Human)

Target Sequence

ASTGLELLGMTLAVLGWLGTLVSCALPLWKVTAFIGNSIVVAQVVWEGLWMSCVVQSTGQMQCKVYDSLLALPQDLQAARALCVIALLLALLGLLVAITGAQCTTCVEDEGAKARIVLTAGVILLLAGILVLIPVCWTAHAIIQDFYNPLVAEALKRELGASLYLGWAAAALLMLGGGLLCCTCPPPQVERPRGPRLGYSIPSRSGASGLDKRDYV

Tag

N-terminal 10xHis-tagged and C-terminal Twin-Strep-tagged

Type

MP-Detergent Transmembrane Protein & Active Protein & In Stock Protein

Source

Mammalian cell

Field of Research

Cancer

Relevance

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) entry into hepatic cells.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Purity

Greater than 95% as determined by SDS-PAGE.

Activity

Yes

Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Human CLDN9 at 2 μg/ml on an Nickel Coated plate can bind Anti-CLDN6/9 recombinant antibody (CSB-RA005508MA1HU) . The EC50 is 2.812-3.524 ng/mL.

Form

Liquid

Buffer

50 mM HEPES, 0.15 M NaCl, 0.05% DDM, 0.01% CHS, pH7.5

Reconstitution

Molecular Weight

27.3 kDa

References & Citations

Claudin-6 and claudin-9 function as additional coreceptors for hepatitis C virus. Zheng A., Yuan F., Li Y., Zhu F., Hou P., Li J., Song X., Ding M., Deng H. J. Virol. 81:12465-12471 (2007)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.