Recombinant Bluetongue virus 10 Core protein VP7 (Segment-7)

Product Specifications

Abbreviation

Recombinant Bluetongue virus 10 Segment-7 protein

Gene Name

Segment-7

UniProt

P69361

Expression Region

1-349aa

Organism

Bluetongue virus 10 (isolate USA) (BTV 10)

Target Sequence

MDTIAARALTVMRACATLQEARIVLEANVMEILGIAINRYNGLTLRGVTMRPTSLAQRNEMFFMCLDMMLSAAGINVGPISPDYTQHMATIGVLATPEIPFTTEAANEIARVTGETSTWGPARQPYGFFLETEETFQPGRWFMRAAQAVTAVVCGPDMIQVSLNAGARGDVQQIFQGRNDPMMIYLVWRRIENFAMAQGNSQQTQAGVTVSVGGVDMRAGRIIAWDGQAALHVHNPTQQNAMVQIQVVFYISMDKTLNQYPALTAEIFNVYSFRDHTWHGLRTAILNRTTLPNMLPPIFPPNDRDSILTLLLLSTLADVYTVLRPEFAIHGVNPMPGPLTRAIARAAYV

Tag

C-terminal 6xHis-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Others

Relevance

The VP7 protein is one of the five proteins (with VP1, VP3, VP4, and VP6) which form the inner capsid of the virus.

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

45.5 kDa

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.