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Recombinant Macaca mulatta Coagulation factor XI (F11) (Active)

Product Specifications

Product Name Alternative

Coagulation factor XI; EGK_16300

Abbreviation

Recombinant Rhesus macaque F11 protein (Active)

Gene Name

F11

UniProt

G7MSF8

Expression Region

19-624aa

Organism

Macaca mulatta (Rhesus macaque)

Target Sequence

ECVTRLFKDIHFEGGDIATVFTPSAKHCQVVCTHHPRCLLFTFTGESASEDPTQWFTCVLKDSVTETLPRVNRTGAISGYSFKQCSHQISACNKDIYVDLDMKGINYNSSLATSAQECQERCTDDVHCHFFTYATRQFPSLEHRNICLLKHTQTGTPTGIMKLDKVVTGFSLKSCALSNLACIRDVFPNTVFADSNIDSVMAPDAFVCRRICTHHPGCLFFTFFSQEWPKESQRNLCLLKTSESGLPRTRIKKSKALSGFSLQSCRHSIPVFCHSSFYHDTDFLGEELDIVAVKGHEACQKLCTNAVRCQFFTYAPAQASCNEGKGKCYLKLSSNGSPTKILRGTGGISGYTLRLCKMDNECTTKIKPRIVGGTASVRGEWPWQVTLHTTSPTQRHLCGGSIIGNQWILTAAHCFYGVESPKILRVYIGILNQSEIKEDTSFFGVQEIIIHDQYKMAESGYDIALLKLETTVNYTDSQRPICLPSKGDRNVIYTDCWVTGWGYRKLRDKIQNTLQKAKIPLVTNDECQKRYRGHKITHKMICAGYREGGKDACKGDSGGPLSCKHNEVWHLVGITSWGEGCAQRERPGVYTNVLEYVDWILEKTQA

Tag

C-terminal 10xHis-tagged

Type

Active Protein & In Stock Protein

Source

Mammalian cell

Field of Research

Cardiovascular

Relevance

/

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Purity

Greater than 95% as determined by SDS-PAGE. Greater than 95% as determined by SEC-HPLC.

Activity

Yes

Bioactivity

①Measured by its binding ability in a functional ELISA. Immobilized Macaca mulatta F11 at 2 μg/mL can bind Anti-F11 recombinant antibody (CSB-RA007916MA1HU) . The EC50 is 0.5654-0.6444 ng/mL. ②Measured by its ability to cleave the fluorogenic peptide substrate, t-butyloxycarbonyl-Ile-Glu-Gly-Arg-7-amido-4-methylcoumarin (Boc-IEGR-AMC) . The specific activity is >150 pmol/min/µg.

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

69.3 kDa

References & Citations

Genome sequencing and comparison of two nonhuman primate animal models, the cynomolgus and Chinese rhesus macaques. Yan G., Zhang G., Fang X., Zhang Y., Li C., Ling F., Cooper D.N., Li Q., Li Y., Wang J. Nat. Biotechnol. 29:1019-1023 (2011)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Protein Length

Full Length of Mature Protein

Available Sizes

Frequently Asked Questions

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