Recombinant Macaca mulatta Coagulation factor XI (F11) (Active)
Product Specifications
Product Name Alternative
Coagulation factor XI; EGK_16300
Abbreviation
Recombinant Rhesus macaque F11 protein (Active)
Gene Name
F11
UniProt
G7MSF8
Expression Region
19-624aa
Organism
Macaca mulatta (Rhesus macaque)
Target Sequence
ECVTRLFKDIHFEGGDIATVFTPSAKHCQVVCTHHPRCLLFTFTGESASEDPTQWFTCVLKDSVTETLPRVNRTGAISGYSFKQCSHQISACNKDIYVDLDMKGINYNSSLATSAQECQERCTDDVHCHFFTYATRQFPSLEHRNICLLKHTQTGTPTGIMKLDKVVTGFSLKSCALSNLACIRDVFPNTVFADSNIDSVMAPDAFVCRRICTHHPGCLFFTFFSQEWPKESQRNLCLLKTSESGLPRTRIKKSKALSGFSLQSCRHSIPVFCHSSFYHDTDFLGEELDIVAVKGHEACQKLCTNAVRCQFFTYAPAQASCNEGKGKCYLKLSSNGSPTKILRGTGGISGYTLRLCKMDNECTTKIKPRIVGGTASVRGEWPWQVTLHTTSPTQRHLCGGSIIGNQWILTAAHCFYGVESPKILRVYIGILNQSEIKEDTSFFGVQEIIIHDQYKMAESGYDIALLKLETTVNYTDSQRPICLPSKGDRNVIYTDCWVTGWGYRKLRDKIQNTLQKAKIPLVTNDECQKRYRGHKITHKMICAGYREGGKDACKGDSGGPLSCKHNEVWHLVGITSWGEGCAQRERPGVYTNVLEYVDWILEKTQA
Tag
C-terminal 10xHis-tagged
Type
Active Protein & In Stock Protein
Source
Mammalian cell
Field of Research
Cardiovascular
Relevance
/
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Purity
Greater than 95% as determined by SDS-PAGE. Greater than 95% as determined by SEC-HPLC.
Activity
Yes
Bioactivity
①Measured by its binding ability in a functional ELISA. Immobilized Macaca mulatta F11 at 2 μg/mL can bind Anti-F11 recombinant antibody (CSB-RA007916MA1HU) . The EC50 is 0.5654-0.6444 ng/mL. ②Measured by its ability to cleave the fluorogenic peptide substrate, t-butyloxycarbonyl-Ile-Glu-Gly-Arg-7-amido-4-methylcoumarin (Boc-IEGR-AMC) . The specific activity is >150 pmol/min/µg.
Form
Lyophilized powder
Buffer
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
69.3 kDa
References & Citations
Genome sequencing and comparison of two nonhuman primate animal models, the cynomolgus and Chinese rhesus macaques. Yan G., Zhang G., Fang X., Zhang Y., Li C., Ling F., Cooper D.N., Li Q., Li Y., Wang J. Nat. Biotechnol. 29:1019-1023 (2011)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Product MSDS
https://www.cusabio.com/msds/12942052/
Protein Length
Full Length of Mature Protein
Available Sizes
Curated Selection
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