Recombinant Human Wnt3a surrogate (Active)

Product Specifications

Product Name Alternative

Abbreviation

Recombinant Human Wnt3a surrogate protein (Active)

Gene Name

Wnt3a surrogate

Expression Region

1-306aa

Organism

Homo sapiens (Human)

Tag

C-terminal hFc1-tagged

Type

Active Protein & In Stock Protein

Source

Mammalian cell

Relevance

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Yes

Bioactivity

Measured by its ability to induce Topflash reporter activity in HEK293T cells. The ED50 for this effect is 17.76-26.50 ng/mL.

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 μm sterile filtered 25 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.2

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

58.5 kDa

References & Citations

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.