Anti-DcR1 (ED2) Antibody

Rabbit Polyclonal Antibody specific to DcR1 (ED2)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Antagonist decoy receptor for TRAIL/Apo-2L, Decoy TRAIL receptor without death domain, Decoy receptor 1, DcR1, Lymphocyte inhibitor of TRAIL, TNF-related apoptosis-inducing ligand receptor 3, TRAIL receptor 3, TRAIL-R3, TRAIL receptor without an intracellular domain, CD antigen CD263

Gene Name

TNFRSF10C

Gene ID

8794

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=TNFRSF10C">TNFRSF10C</a>

UniProt

O14798

Accession Number

NP_003832

Cellular Locus

Cell membrane; Lipid-anchor, GPI-anchor.

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Peptide corresponding to aa 111- 123 at the extracellular domain (ED) of human DcR1 precursor (accession no. AAB67104).

Target Antigen

Tumor necrosis factor receptor superfamily member 10C

Target

DcR1 (ED2)

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, IF

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Receptor for the cytotoxic ligand TRAIL. Lacks a cytoplasmic death domain and hence is not capable of inducing apoptosis. May protect cells against TRAIL mediated apoptosis by competing with TRAIL-R1 and R2 for binding to the ligand.

Additionnal Information

Immunoblotting: use at 1ug/mL. Immunofluorescence: use at 10ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: Whole cell lysate from HeLa cells.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human, mouse, and rat DcR1 (65kDa).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

TRAIL/Apo2L is a new member of the TNF family that induces apoptosis in a variety of tumor cell lines. DR4 and DR5 are the recently identified functional receptors for TRAIL. Two decoy receptors for TRAIL have been designated DcR1/TRID/TRAIL- R3/LIT and DcR2/TRAIL-R4/TRUNDD. DcR1 has an extracellular TRAIL-binding domain but lacks an intracellular signaling domain. It is a glycophospholipid-anchored cell surface protein. DcR1 transcripts are expessed in many normal human tissues but not in most cancer cell lines. Overexpression of DcR1 does not induce apoptosis but attenuates TRAIL-induced apoptosis.