Anti-Id3 Antibody

Class B basic helix-loop-helix protein 25, bHLHb25, Helix-loop-helix protein HEIR-1, ID-like protein inhibitor HLH 1R21, Inhibitor of DNA binding 3, Inhibitor of differentiation 3

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Involved in myogenesis by inhibiting skeletal muscle and cardiac myocyte differentiation and promoting muscle precursor cells proliferation. Inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. {PubMed:8437843}.

Immunoblotting and Immuno- precipitation: use at 1-5 ug/mL. <br><br>Positive controls: Id3 transfected Sol8 muscles cells and recombinant fusion protein.

Id3 is a 14 kD protein and a member of the Id family of helix-loop-helix (HLH) proteins. Id proteins are believed to be dominant negative regulators of HLH involved in cell differentiation and implicated in the control of cell growth, cellular differentiation, apoptosis, and carcinogenesis.