ELISA Kit for Pituitary Adenylate Cyclase Activating Peptide (PACAP)

This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to ADCYAP1 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled ADCYAP1 and unlabeled ADCYAP1 (Standards or samples) with the pre-coated antibody specific to ADCYAP1. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of ADCYAP1 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of ADCYAP1 in the sample.

1. Prepare all reagents, samples and standards; <br/> 2. Add 50&micro; L standard or sample to each well.<br/> &nbsp; &nbsp; &nbsp; &nbsp; And then add 50&micro; L prepared Detection Reagent A immediately.<br/> &nbsp; &nbsp; &nbsp; &nbsp; Shake and mix. Incubate 1 hour at 37&deg; C; <br/> 3. Aspirate and wash 3 times; <br/> 4. Add 100&micro; L prepared Detection Reagent B. Incubate 30 minutes at 37&deg; C; <br/> 5. Aspirate and wash 5 times; <br/> 6. Add 90&micro; L Substrate Solution. Incubate 10-20 minutes at 37&deg; C; <br/> 7. Add 50&micro; L Stop Solution. Read at 450 nm immediately.<br/>

<p> Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Pituitary Adenylate Cyclase Activating Peptide (PACAP) were tested 20 times on one plate, respectively. <br/> Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Pituitary Adenylate Cyclase Activating Peptide (PACAP) were tested on 3 different plates, 8 replicates in each plate. <br/> CV (%) = SD/meanX100 <br/> Intra-Assay: CV<10% <br/> Inter-Assay: CV<12% <br/> </p>

<p> The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. <br/> To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. </p>

<p> This assay has high sensitivity and excellent specificity for detection of Pituitary Adenylate Cyclase Activating Peptide (PACAP) .<br/> No significant cross-reactivity or interference between Pituitary Adenylate Cyclase Activating Peptide (PACAP) and analogues was observed. </p>