ELISA Kit for Calprotectin (CALPRO)

The microplate provided in this kit has been pre-coated with an antibody specific to S100A8. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to S100A9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain calprotectin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of calprotectin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

1. Prepare all reagents, samples and standards; <br/> 2. Add 100&micro; L standard or sample to each well. Incubate 2 hours at 37&deg; C; <br/> 3. Aspirate and add 100&micro; L prepared Detection Reagent A. Incubate 1 hour at 37&deg; C; <br/> 4. Aspirate and wash 3 times; <br/> 5. Add 100&micro; L prepared Detection Reagent B. Incubate 30 minutes at 37&deg; C; <br/> 6. Aspirate and wash 5 times; <br/> 7. Add 90&micro; L Substrate Solution. Incubate 10-20 minutes at 37&deg; C; <br/> 8. Add 50&micro; L Stop Solution. Read at 450nm immediately. <br/>

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Calprotectin (CALPRO) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Calprotectin (CALPRO) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

This assay has high sensitivity and excellent specificity for detection of Calprotectin (CALPRO) . No significant cross-reactivity or interference between Calprotectin (CALPRO) and analogues was observed.