Rapid ELISA Kit for High Mobility Group Protein 1 (HMGB1)

The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant High Mobility Group Protein 1 (HMGB1) . Standards or samples and HRP-labeled detection antibody specific to Instant High Mobility Group Protein 1 (HMGB1) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant High Mobility Group Protein 1 (HMGB1), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Instant High Mobility Group Protein 1 (HMGB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

1. Prepare all reagents, samples and standards; <br/> 2. Add 100&micro; L standard or sample to each well. Incubate 2 hours at 37&deg; C; <br/> 3. Aspirate and add 100&micro; L prepared Detection Reagent A. Incubate 1 hour at 37&deg; C; <br/> 4. Aspirate and wash 3 times; <br/> 5. Add 100&micro; L prepared Detection Reagent B. Incubate 30 minutes at 37&deg; C; <br/> 6. Aspirate and wash 5 times; <br/> 7. Add 90&micro; L Substrate Solution. Incubate 10-20 minutes at 37&deg; C; <br/> 8. Add 50&micro; L Stop Solution. Read at 450nm immediately. <br/>

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Instant High Mobility Group Protein 1 (HMGB1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Instant High Mobility Group Protein 1 (HMGB1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

This assay has high sensitivity and excellent specificity for detection of Instant High Mobility Group Protein 1 (HMGB1) . No significant cross-reactivity or interference between Instant High Mobility Group Protein 1 (HMGB1) and analogues was observed.