Human SMN1 Protein, His Tag

SMN1 is produced chiefly by the SMN1 gene, located on the telomeric portion of chromosome 5q. SMN1 protein is one of a group of proteins called the SMN complex, which is important for the maintenance of specialized nerve cells called motor neurons. In cells, the SMN complex plays an important role in processing mRNA. The SMN complex helps to assemble the cellular machinery needed to process pre-mRNA. The SMN complex is also important for the development of specialized outgrowths from nerve cells called dendrites and axons. Deletion or mutation of the SMN1 gene results in a reduced level of full-length SMN protein and manifests as a range of neuromuscular phenotypes in humans as the disease spinal muscular atrophy (SMA) .

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 33.7 kDa. The protein migrates as 36-40 kDa and 69 kDa under reducing (R) condition (SDS-PAGE) .

Accession Number

Q16637-1

Expression Region

Ala 2 - Asn 294

Host

E. coli

Target

SMN1

Conjugation

Unconjugated

Tag

N-10xHis

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human SMN1, His Tag (Cat. No. SM1-H5145) at 1 μg/mL (100 μL/well) can bind Anti-SMN1 antibody with a linear range of 0.2-8 ng/mL (QC tested).