What is the catalog number for ELISA Kit for D-Dimer (D2D)?

The catalog number for ELISA Kit for D-Dimer (D2D) is CEA506Po.

Is ELISA Kit for D-Dimer (D2D) in stock?

ELISA Kit for D-Dimer (D2D) is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures ELISA Kit for D-Dimer (D2D)?

ELISA Kit for D-Dimer (D2D) is manufactured by Torvigen.

What is the estimated delivery time for ELISA Kit for D-Dimer (D2D)?

Estimated delivery time for ELISA Kit for D-Dimer (D2D) is approximately 15 business day(s), depending on your location.

Is this product animal-origin-free?

Animal-origin-free status, where applicable, is noted on the datasheet under formulation details.

Does this product need any special customs documentation to be imported to my country?

If you’re outside the EU, UK, or USA, you’re responsible for customs clearance and any related taxes or fees. We provide the standard import documents, such as the Commercial Invoice, Packing List, and Airway Bill, in advance, so let us know early if your customs office needs anything additional for this specific product.

What buffer is this product supplied in?

The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.

ELISA Kit for D-Dimer (D2D)

Product Specifications

CAS Number

7732-18-5

Product Name Alternative

D 2 Dimer

Applications

Enzyme-linked immunosorbent assay for Antigen Detection.

Assay Principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to D-Dimer (D2D) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled D-Dimer (D2D) and unlabeled D-Dimer (D2D) (Standards or samples) with the pre-coated antibody specific to D-Dimer (D2D) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of D-Dimer (D2D) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of D-Dimer (D2D) in the sample.

Assay Protocol

1. Prepare all reagents, samples and standards; 2. Add 50µ L standard or sample to each well.         And then add 50µ L prepared Detection Reagent A immediately.         Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C; 7. Add 50µ L Stop Solution. Read at 450 nm immediately.

Assay Performance Time

Sample Type

Plasma

Detection Range

617.3 - 50000 pg/mL

Precision

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level D-Dimer (D2D) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level D-Dimer (D2D) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

Sensitivity

The minimum detectable dose of this kit is typically less than 237.3pg/mL

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Specificity

This assay has high sensitivity and excellent specificity for detection of D-Dimer (D2D) . No significant cross-reactivity or interference between D-Dimer (D2D) and analogues was observed.