Recombinant Severe acute respiratory syndrome coronavirus 2 Spike glycoprotein (S), partial (Active)
Product Specifications
Product Name Alternative
/
Abbreviation
Recombinant SARS-CoV-2 S protein, partial (Active)
Gene Name
S
UniProt
P0DTC2
Expression Region
319-541aa
Organism
Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Target Sequence
RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF
Tag
C-terminal 6xHis-mFc-tagged
Type
Active Protein & In Stock Protein & Coronavirus Protein
Source
Mammalian cell
Field of Research
Microbiology
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Yes
Bioactivity
①Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind Biotinylated Anti-SARS-CoV-2-S Antibody (CSB-RA33245D1GMY), the EC50 of SARS-CoV-2-S1-RBD protein is 106.2-131.2 ng/ml. ②Measured by its binding ability in a functional ELISA. Immobilized human ACE2 (CSB-MP866317HU) at 2 μg/ml can bind SARS-CoV-2-S1-RBD, the EC50 of SARS-CoV-2-S1-RBD protein is 8.363-12.82 ng/ml.
Form
Lyophilized powder
Buffer
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
51.1 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Partial
Available Sizes
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