Anti-Platelet GMP-140 (P-selectin/CD62) Antibody

Mouse Monoclonal Antibody specific to Human Platelet GMP-140 (P-selectin/CD62)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

CD62 antigen-like family member P, Granule membrane protein 140, GMP-140, Leukocyte-endothelial cell adhesion molecule 3, LECAM3, Platelet activation dependent granule-external membrane protein, PADGEM, CD antigen CD62P

Gene Name

SELP

NCBI Gene ID

UniProt

P16109

Cellular Locus

Cell membrane, Single-pass type I membrane protein

Host

Mouse

Reactivity

Human

Immunogen

Human platelets

Target Antigen

P-selectin

Target

Platelet GMP-140 (P-selectin/CD62)

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, IP, FACS, FC

Field of Research

Cell adhesion

Purification Method

Purified by immunoaffinity chromatography

Dilution

Reconstitute lyophilized antibody in 50ul of distilled water (2.0mg/ml final concentration).

Format

Purified

Form

Lyophilized

Buffer

Phosphate Buffered Saline

Function

Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand rPubMed:11081633, PubMed:28011641, PubMed:7585950}.

Additionnal Information

ELISA: 1-10ug/ml with immobilized antigen.Flow cytometry: 1-10ug/mlWestern Blot: 5-10ug/ml, non-reducing and non-heating conditions. <br><br>Immunohistochemistry: 5-10ug/ml, frozen tissue sections.

Storage Conditions

This product does not contain preservative. Lyophilized antibody is stable at 4°C for 2 years. Store the reconstituted solution (2. 0mg/ml) in aliquots at -20°C for one year or at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles.

Specificity

This antibody reacts with GMP-140 (P- selectin) on activated human platelets; it does not react with resting platelets. It does not interfere with platelet aggregation or platelet adhesion. It does not cross-react with rabbit, rat, porcine, or canine activated platelets.