Recombinant Human Angiotensin-converting enzyme 2 (ACE2), partial (Active)

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①Measured by ACE2 protein (CSB-MP866317HU) binding ability in a functional ELISA. Coat the plate with 0.2μg/well (2μg/ml, 100μl/well) of SARS-CoV-2 S1 protein (CSB-MP3324GMY), add 100μl/well (0.7629-100000ng/ml, gradient dilution, 2x to 18x) of ACE2 protein and 100μl/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific (CSB-PA160506) . Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1 protein could bind ACE2 protein. The EC50 is 56.64 - 103.6 ng/ml. ②Measured by ACE2 protein (CSB-MP866317HU) binding ability in a functional ELISA. Coat the plate with 0.5μg/well (5μg/ml, 100μl/well) of SARS-CoV-2 S1-RBD protein (CSB-YP3324GMY1), add 100μl/well (0.762939-100000ng/ml, gradient dilution, 2x to 18x) of ACE2 protein and 100μl/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific (CSB-PA160506) . Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1-RBD protein could bind ACE2 protein. The EC50 is 31.80 - 44.69 ng/ml. ③Measured by ACE2 protein (CSB-MP866317HU) binding ability in a functional ELISA. Coat the plate with 0.5μg/well (5μg/ml, 100μl/well) of SARS-CoV-2 S1-RBD protein (CSB-MP3324GMY1b1), add 100μl/well (0.762939-100000ng/ml, gradient dilution, 2x to 18x) of ACE2 protein and 100μl/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific (CSB-PA160506) . Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1-RBD protein could bind ACE2 protein. The EC50 is 2.785-9.139 ng/ml. ④Measured by ACE2 protein (CSB-MP866317HU) binding ability in a functional ELISA. Coat the plate with 0.2μg/well (2μg/ml, 100μl/well) of ACE2 protein, add 100μl/well (0.00001164153-200000ng/ml, gradient dilution, 4x to 18x) of SARS-CoV-2 S1-RBD protein (CSB-MP3324GMY1) and 100μl/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific (CSB-PA160506) . Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1-RBD protein could bind ACE2 protein. The EC50 is 8.363-12.82 ng/ml ng/ml. ⑤The SPR sensorgram shows the binding between ACE2 protein (CSB-MP866317HU) and SARS-CoV-2 S1-RBD protein (CSB-YP3324GMY1) . ACE2 protein was injected at 5 concentrations (400, 200, 100, 50, 0 nM) across 0.5 mg/ml of SARS-CoV-2 S1-RBD protein captured on a COOH chip. The results showed ACE2 protein could bind SARS-CoV-2 S1-RBD protein with an affinity constant of 100 nM. ⑥The SPR sensorgram shows the binding between ACE2 protein (CSB-MP866317HU) and SARS-CoV-2 S1-RBD protein (CSB-MP3324GMY1b1) . ACE2 protein was injected at 5 concentrations (100, 50, 25, 12.5, 0 nM) across 0.5 mg/ml of SARS-CoV-2 S1-RBD protein captured on a COOH chip. The results showed ACE2 protein could bind SARS-CoV-2 S1-RBD protein with an affinity constant of 13.8 nM.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.