Recombinant Human Macrophage Inflammatory Protein-1 beta/CCL4
Product Specifications
Background
CCL4 encoded by the CCL4 gene, also known as Macrophage Inflammatory Protein-1 beta; (MIP-1 beta; ) is a CC chemokine with specificity for CCR5 receptors and it is a major HIV-suppressive factor produced by CD8+ T cells. It is a monokine with inflammatory and chemokinetic properties. Recombinant CCL4 induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV) . Specifically, MIP-1-beta (3-69) is also a ligand for CCR1 and CCR2 isoform B. Recombinant human CCL4 contains 69 amino acids and it shares 77 % and 80 % a.a. sequence identity with murine and rat CCL4, respectively. Both human and murine MIP-1 alpha; and MIP-1 beta; are active on human and murine hematopoietic cells.
Product Name Alternative
G-26 T-lymphocyte-secreted protein, HC21, LAG-1, MIP-1-beta, PAT 744, Protein H400, SIS-gamma, Small-inducible cytokine A4, ACT-2
Gene ID
6351
UniProt
P13236
Target
APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRSKQV CADPSESWVQ EYVYDLELN
Purification
> 96 % by SDS-PAGE and HPLC analyses.
Endotoxin
Less than 1 EU/ug of rHuMIP-1β/CCL4 as determined by LAL method.
Bioactivity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 5.0-20 ng/ml.
Storage Conditions
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculated Molecular Weight
Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in PBS.
Host or Source
Escherichia coli
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