Terminal Transferase (TdT)
Terminal transferase (TdT) is a template independent polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules. It catalyzes the repeated addition of deoxyribonucleotides to oligodeoxyribonucleotides and 3' -Oh of single - and double-stranded DNA. TdT requires an oligodeoxynucleotide of at least three bases as a primer. The addition of Co2+ in the reacton makes tailing more efficient.
Product Specifications
Product Name Alternative
DNA nucleotidylexotransferase, 2.7.7.31, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, TDT, Terminal transferase, DNTT, TDT
Nature
Recombinant
Applications
Terminal transferase catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules.
Endotoxin
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Form
Liquid
Buffer
100mM KAc (pH6.8), 2mM 2-mercaptoethanol, 0.01%Triton X-100 and 50% glycerol.
Shipping Conditions
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Storage Conditions
Notes
For research use only.
Calculated Molecular Weight
45 kDa
Protein Length
Terminal Transferase (TdT) is expressed in E. coli.
Species
Bovine
Available Sizes
Frequently Asked Questions
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