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PNGase F

Peptide -N-Glycosidase F, also known as PNGase F, is an amidase that cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins

Product Specifications

Expression System

E. coli

Nature

Recombinant

Applications

Removal of high mannose N-glycans from glycoproteins

Endotoxin

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Form

Liquid

Buffer

20mM Tris-HCl, pH 7.5, 50mM NaCl, 5mM EDTA, 50% glycerol

Shipping Conditions

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Storage Conditions

Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.

Notes

For research use only.

Calculated Molecular Weight

37.08 kDa

Protein Length

PNGase F is cloned from Elizabethkingia miricola and expressed in E.coli.

Species

Elizabethkingia miricola

Available Sizes

Frequently Asked Questions

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