Anti-MPXV A35R Human IgG ELISA Kit

PRINCIPLE OF THE ASSAY This assay employs the quantitative indirect enzyme immunoassay technique. Recombinant MPXV A35R antigen has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Human Anti-MPXV A35R IgG present is bound by the immobilized protein. After washing away any unbound substances, a biotin-labeled Mouse Anti-Human IgG antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Human Anti-MPXV A35R IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.