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SARS-CoV-2 RBD ELISA Kit

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for SARS-CoV-2 RBD has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any SARS-CoV-2 RBD present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled SARS-CoV-2 RBD antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells.Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SARS-CoV-2 RBD bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Specifications

Detection Method

Colorimetric

Assay Type

Quantitative

Sample Type

Serum, Plasma and Cell culture supernatant.

Detection Range

12.5 - 800 pg/mL

Recovery

80-120 %

Sensitivity

4.92 pg/mL

Shipping Conditions

2-8 °C

Storage Conditions

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20°C, the rest reagents should be store at 4°C.
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