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Anti-Omalizumab Neutralizing Antibody ELISA kit

PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Omalizumab has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled Human IgE and then pipetted into the wells. Anti-Omalizumab Neutralizing Antibody in the sample competitively binds to Omalizumab with the biotin-labeled Human IgE. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Anti-Omalizumab Neutralizing Antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Specifications

Specifications

Omalizumab

Product Name Alternative

IGE25, olizumab, pSVIE26, rhuMab-E25, CAS: 242138-07-4

Detection Method

Colorimetric

Assay Type

Quantitative

Sample Type

Plasma, Serum

Detection Range

156.25 - 10,000 ng/mL

Recovery

80-120%

Sensitivity

82.6 ng/mL

Shipping Conditions

2-8 °C

Storage Conditions

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20°C, the rest reagents should be store at 4°C.

Notes

For Research Use Only.

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