Romosozumab ELISA Kit

PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Human SOST has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Romosozumab in the sample competitively binds to the pre-coated protein with biotin-labeled Romosozumab. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Romosozumab bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Specifications

Background

Romosozumab, a specific inhibitor of sclerostin, is a unique approach to therapy for postmenopausal osteoporosis and related disorders. The elucidation of sclerostin deficiency as the molecular defect of syndromes of high bone mass with normal quality, and the pivotal role of sclerostin as a mediator of osteoblastic activity and bone formation, provided the platform for the evaluation of inhibitors of sclerostin to activate bone formation.

Specifications

Romosozumab

Product Name Alternative

785A070802, AMG 785, CDP-7851, romosozumab-aqqg, sclerostin Ab, CAS: 909395-70-6

Detection Method

Colorimetric

Assay Type

Quantitative

Sample Type

Plasma, Serum

Detection Range

125 - 8,000 ng/mL

Recovery

80-120%

Sensitivity

42.86 ng/mL

Shipping Conditions

2-8 °C

Storage Conditions

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20°C, the rest reagents should be store at 4°C.