Ranibizumab ELISA Kit
PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant human VEGFA antigen has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Ranibizumab in the sample competitively binds to the pre-coated protein with biotin-labeled Ranibizumab. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Ranibizumab bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Specifications
Background
Product Name Alternative
Fab-12 variant Y0317, RhuFab, CAS: 347396-82-1
Detection Method
Colorimetric
Assay Type
Quantitative
Sample Type
Plasma, Serum
Detection Range
234.4 ng/mL - 15,000 ng/mL
Recovery
80-120 %
Shipping Conditions
2-8 °C
Storage Conditions
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