Trastuzumab ELISA Kit

PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. A recombinant human ErbB2 antigen has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells.Trastuzumab in the sample competitively binds to the pre-coated protein with biotin-labeled Trastuzumab. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Trastuzumab bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Specifications

Product Name Alternative

Timigutuzumab, 4D5-8,4D5V8, Herceptin, rhuMab HER2, CAS: 180288-69-1

Detection Method

Colorimetric

Assay Type

Quantitative

Sample Type

Plasma, Serum

Detection Range

78.13 - 5,000 ng/mL

Recovery

80-120 %

Sensitivity

70.12 ng/mL

Shipping Conditions

2-8 °C

Storage Conditions

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20°C, the rest reagents should be store at 4°C.