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REACH Cyprinid Vitellogenin ELISA (TECO®)

The REACH Cyprinid Vitellogenin ELISA Kit is a sensitive sandwich enzyme linked immunosorbent assay for the quantitative determination of vitellogenin in serum, WBH and mucus of cyprinids according to EC regulation Nr. 440/2008 (REACH) from July 10th 2015/ Document D039048/03

Product Specifications

Sample Volume

50 µL

Detection Range

0.4-70 ng/mL

Sensitivity

LLOQ 0.04 ng/mL LLD 0.025 ng/mL

Components

Standards:       Standard Stock, lyophilized, 2 vials Controls: 3 Control levels, lyophilized, 2 vials each Inter-Assay Reference Standard Stock, lyophilized, 1 vial NSB-Strip:      1 breakable strip of 8 wells Sample preparationSerum: Store fresh serum samples immediately after collection at -20°C or lower until assayed. WBH: Store fresh WBH samples immediately after collection at -20°C or lower until assayed. Collect mucus as described in the TECO® Mucus Collection Set (TE1034) . Mucus-containing swabs can be stored several months at <-20°C Reference valuesSerum levels are in the range of µg/mL up to mg/mL WBH levels are in the range of mg/mL Mucus level are in the range of ng/mL

Species

Fish Cyprinid Carp (Caprinus carpio) Goldfish (Carassius auratus) Zebrafish (Danio rerio) Fathead Minnow (Pimephales promelas)

Incubation Time

4.0 Hours

Frequently Asked Questions

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