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REACH Medaka Vitellogenin ELISA (TECO®)

The REACH Medaka Vitellogenin ELISA Kit is a sensitive sandwich enzyme linked immunosorbent assay for the quantitative determination of vitellogenin in serum, WBH and mucus of Medaka according to EC regulation Nr. 440/2008 (REACH) from July 10th 2015/ Document D039048/03

Product Specifications

Sample Volume

50 µL

Detection Range

2.6 -210 ng/mL

Sensitivity

LLOQ < 2.6 ng/mL LLD 0.7 ng/mL

Components

Standards: Standard Stock, lyophilized, 2 vials Controls: 2 Control levels, lyophilized, 2 vials each Inter-Assay Reference Standard Stock, lyophilized, 1 vial NSB-Strip: 1 breakable strip of 8 wells Sample preparationSerum: Store fresh serum samples immediately after collection at -20°C or lower until assayed. WBH: Store fresh WBH samples immediately after collection at -20°C or lower until assayed. Collect mucus as described in the TECO® Mucus Collection Set (TE1034) . Mucus-containing swabs can be stored several months at <-20°C Reference valuesSerum levels are in the range of µg/mL up to mg/mL WBH levels are in the range of mg/mL Mucus level are in the range of ng/mL

Species

Fish Medaka (Oryzias latipes)

Incubation Time

4.0 Hours

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