REACH Perch (Perciformes) Vitellogenin ELISA (TECO®)
The Perch (Perciformes) Vitellogenin ELISA Kit is a sensitive sandwich enzyme linked immunosorbent assay for the quantitative determination of vitellogenin in serum and mucus of perciformes according to EC regulation Nr. 440/2008 (REACH) from July 10th 2015/ Document D039048/03.
Product Specifications
Sample Volume
50 µL
Detection Range
1-80 ng/mL
Sensitivity
LLOQ < 1.0 ng/mL LLD < 0.22 ng/mL
Components
Standards: Standard Stock, lyophilized, 2 vials Controls: 2 Control levels, lyophilized, 2 vials each Inter-Assay Reference Standard Stock, lyophilized, 1 vial NSB-Strip 1 breakable strip of 8 wells Sample preparationSerum: Store fresh serum samples immediately after collection at -20°C or lower until assayed. Mucus: Collect mucus as described in the TECO® Mucus Collection Set (TE1034) . Mucus-containing swabs can be stored several months at <-20°C Reference valuesSerum levels are in the range of µg/mL up to mg/mL Mucus level are in the range of ng/mL
Species
Fish Tilapia (Oreochromis niloticus) Bluegill (Lepomis macrochirus) “European perch/perch/redfin perch/English perch" (Perca fluviatilis) Ruffe (Gymnocephalus cernua) Three-spined stickleback (Gasterosteus aculeatus)
Incubation Time
4.0 Hours
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