SSEMA4D

Intended use: Semaphorin 4D (SEMA4D or CD100) is a member of a family of transmembrane and secreted proteins that regulates key cellular functions and is involved in cell-cell communication (1-3) . Most of the effects of SEMA4D is mediated by plexins (4,5) . SEMA4D participates in numerous physiological processes such as axon guidance, immune regulation, angiogenesis, tumor progression, and bone metabolism (6-9) . Cleavage of SEMA4D near the cell membrane through matrix metalloproteinases leads to the biologically active soluble SEMA4D with a molecular weight of 120 kD consisting of 713 amino acids (3,5, 10) . SEMA4D has emerged to a novel therapeutic target in cancer and in bone diseases (11,12) . Semaphorin 4D is widely studied for ist role in neural connectivity, vascularization, cell migration, the immune response, tumor progression, and bone remodeling. This sSEMA4D ELISA utilizes two monoclonal anti-human Semaphorin 4D antibodies, both recognizing conformational epitopes on Semaphorin 4D. The epitopes have been mapped by overlapping cyclic peptides and shown to involve amino acids AA30-AA34 and amino acids AA238-AA241, respectively (for further information on antibody characterization please visit our website www.bmgrp.com: see Validation Data) Intended applications: Osteology Neurology Immunology Oncology