M65® ELISA (PEVIVA®)
Quantitative measurement of total soluble cytokeratin 18 (K18) released from dead cells (necrotic and apoptotic) . The cells or tissues should be of human epithelial origin (e.g. kidney, gut, colon, lung or liver) expressing K18. The M65® ELISA can be combined with the M30-Apoptosense® ELISA (PEVIVA Prod. No. 10010) for determination of cell death mode (apoptosis versus necrosis) . Death mode can be determined in vitro and in serum from cancer patients (Kramer et al., Cancer Res. 2004) .
Product Specifications
Assay Type
ELISA
Sample Volume
25 µL
Detection Range
125 - 2000 U/L (The Units measured by the M65® ELISA are defined against a synthetic standard. 1 U/L = 1.24 pM.)
Sensitivity
25 U/L
Species
Human, primates, bovine
Incubation Time
2 Hours 20 minutes
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