M30 Apoptosense® ELISA (PEVIVA®)
The M30 neo-epitope (K18Asp396-NE) is a sensitive and integrative indicator specific for epithelial cell death involving caspase -3, -7 or -9 activation. Quantitative measurement of the apoptotic cell death biomarker K18Asp396-NE (“M30 antigen”) released from dying human epithelial cell lines in vitro or in vivo. The cells or tissues should be of human epithelial origin (e.g. kidney, gut, colon, lung or liver) expressing K18. * Note: caspase-cleaved K18 = ccK18 previously Cytokeratin 18 (CK18/ccCK18)
Product Specifications
Assay Type
ELISA
Sample Volume
25 µL
Detection Range
40 – 1000 U/l (units are defined against a synthetic peptide standard containing the M30 and M5 epitopes; 1 U/l = 1.24 pM)
Sensitivity
20 U/L
Species
Human, Primates
Incubation Time
4.5 Hours
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