Streptavidin (A24C) Protein, His Tag

Streptavidin is a 66KDa tetrameric protein purified from the bacterium Streptomyces avidinii, and exhibits high binding affinity to biotin. Each unit can bind one biotin. Horseradish peroxidase is metalloenzyme, a 44KDa glycoprotein. When incubate with substrates, it produces a coloured, fluorimetric, or luminescent derivatives, which can be detected and quantified. HRP conjugated Streptavidin is widely used for the detection and quantification of biotinylated proteins.

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 18.6 kDa. The protein migrates as 20-22 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) .

● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Immobilized Biotinylated Human Dkk-1, Fc,Avitag (Cat. No. DK1-H82F5) at 5 μg/mL (100 μL/well) on Streptavidin (A24C) Protein, His Tag (Cat. No. STN-N51H3) precoated (0.5 μg/well) plate can bind Human LRP-5, Mouse IgG2a Fc Tag (Cat. No. LR5-H5254) with a linear range of 0.5-16 ng/mL (QC tested).