Recombinant Human GCLC Protein (Trx Tag)
Product Specifications
Background
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.
Abbreviation
GCLC
Synonyms
Glutamate-cysteine ligase catalytic subunit; Gamma-ECS; GCS heavy chain
UniProt
P48506
Accession Number
P48506
Expression System
E.coli
Tag
N-Trx
Sequence
Met1-Val252
Field of Research
Metabolism; Cell Biology; Cancer
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method
Purity
> 95% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
47.6 kDa
Observed Molecular Weight
47 kDa
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
Species
Human
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
BNC400095-100 1x 100 µL
Nucleolar / Nucleoli (NM95), CF640R conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
RD-QPCT-Hu-01 96 Tests
Human Glutaminyl Peptide Cyclotransferase (QPCT) ELISA Kit
Sign In for Pricing
View Details RD-QPCT-Hu-02 48 Tests
Human Glutaminyl Peptide Cyclotransferase (QPCT) ELISA Kit
Sign In for Pricing
View Details 
