Recombinant Rabbit TNFα protein (His tag)
Product Specifications
Background
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance.Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.
Abbreviation
TNFA; TNFα
Synonyms
Cachectin; ICD1; ICD2; N-terminal fragment; NTF; TNF-a; TNF-alpha; Tumor necrosis factor ligand superfamily member 2
UniProt
P04924
Accession Number
P04924
Expression System
HEK293 Cells
Tag
C-His
Sequence
Gly57-Leu235
Endotoxin
< 1.0 EU/mg of the protein as determined by the LAL method.
Purity
> 90% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Product Datasheet
https://789.bio/ea/pcorx5
Product MSDS
https://789.bio/eb/DxyDxP
Calculated Molecular Weight
20.0 kDa
Observed Molecular Weight
18 kDa
Species
Rabbit
Available Sizes
Curated Selection
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