Recombinant Mouse IL6 protein (His tag)

Product Specifications

Background

Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism (Probable) . Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway.The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.

Abbreviation

IL6

Synonyms

BSF; Interleukin; Interleukin HP; IFNB; B-cell hybridoma growth factor; BSF2; CDF; HGF; HSF; IFNB2; IL 6; Interleukin HP-1; Interleukin-6; Il-6; Il6

UniProt

P08505

Accession Number

P08505

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met1-Thr211

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.