Recombinant Human IGFBP3 protein (His tag)

Product Specifications

Background

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. Inhibits the positive effect of humanin on insulin sensitivity.Promotes testicular germ cell apoptosis.

Abbreviation

IGFBP3

Synonyms

IBP-3; IGF-binding protein 3; IGFBP-3

UniProt

P17936

Accession Number

P17936

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met1-Lys291

Field of Research

Cell Biology, Signal Transduction, Cardiovascular, Metabolism, Cancer

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 80% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.