Recombinant Rat IGFBP1/IGFBP-1 protein (His tag)

Product Specifications

Background

The insulin-like growth factor binding protein (IGFBP) family consists of six structurally related proteins that bind IGF with high affinity. These proteins share conserved cysteine-rich N-and C-terminal regions that participate in IGF binding. IGFBPs regulate the bioavailability of IGFs and modulate their biological activities, both positively and negatively. Some IGFBPs also have intrinsic bioactivity that is IGF-independent. Post-transitional modifications of the IGFBPs, including glycosylation, phosphorylation and proteolysis, influence IGF binding affinities and tissue localization, affecting both the IGF-dependent and independent functions.

Abbreviation

IGFBP1; IGFBP-1

Synonyms

IBP-1; IGF-binding protein 1; IGFBP-1; Igfbp1; Insulin-like growth factor-binding protein 1

UniProt

P21743

Accession Number

P21743

Expression System

E.coli

Tag

N-His

Sequence

Ala 26-Asn 272

Field of Research

Cardiovascular; signal Transduction

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.