Recombinant rat Prostatic Acid Phosphatase/ACPP protein (His tag)

Product Specifications

Background

ACPP (Acid phosphatase, prostate, also PAP and ACP3) is a 48-52 kDa glycoprotein member of the histidine acid phosphatase family of enzymes. It exists as a 95-100 kDa nondisulfide-linked homodimer that hydrolyzes phosphate esters under low pH to generate free phosphate. ACPP is expressed by prostate epithelium and pain-detecting spinal cord neurons. In the spinal cord, ACPP dephosphorylates AMP.

Abbreviation

ACPP

Synonyms

Acid phosphatase 3; Acp3; Fluoride-resistant acid phosphatase; Prostatic acid phosphatase; Protein tyrosine phosphatase ACP3; Thiamine monophosphatase (TMPase)

UniProt

P20646

Accession Number

P20646

Expression System

E.coli

Tag

N-His

Sequence

Lys 32-Leu 381

Field of Research

Cancer; Cell Biology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.