Recombinant Rat ACE protein (His tag)
Product Specifications
Background
Dipeptidyl carboxypeptidase that removes dipeptides from the C-terminus of a variety of circulating hormones, such as angiotensin I, bradykinin or enkephalins, thereby playing a key role in the regulation of blood pressure, electrolyte homeostasis or synaptic plasticity. Plays a major role in the angiotensin-renin system that regulates blood pressure and sodium retention by the kidney by converting angiotensin I to angiotensin II, resulting in an increase of the vasoconstrictor activity of angiotensin.
Abbreviation
ACE
Synonyms
Angiotensin-converting enzyme; CD143; Dcp1; Dipeptidyl carboxypeptidase I; Kininase II
UniProt
P47820
Accession Number
P47820
Expression System
E.coli
Tag
N-His
Sequence
Phe 1071-Ser 1313
Field of Research
Cardiovascular; Signal Transduction; Stem Cell; Cell Biology; Cardiovascular; Cancer; Metabolism
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method.
Purity
> 95% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
26.6 kDa
Observed Molecular Weight
28 kDa
Species
Rat
Available Sizes
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