Recombinant Rat GPRC6A protein (His tag)

Product Specifications

Background

Receptor activated by amino acids with a preference for basic amino acids such as L-Lys, L-Arg and L-ornithine but also by small and polar amino acids. The L-alpha amino acids respond is augmented by divalent cations Ca (2+) and Mg (2+) . Activated by extracellular calcium and osteocalin. Seems to act through a G (q) /G (11) and G (i) -coupled pathway. Mediates the non-genomic effects of androgens in multiple tissue. May coordinates nutritionnal and hormonal anabolic signals through the sensing of extracellular amino acids, osteocalcin, divalents ions and its responsiveness to anabolic steroids.

Abbreviation

GPRC6A

Synonyms

G protein coupled receptor 33; G-protein coupled receptor family C group 6 member A; GPC6A; GPCR33; Predicted with SOSUI analysis; bA86F4.3; seven transmembrane helix receptor

UniProt

Q70VB1

Accession Number

Q70VB1

Expression System

E.coli

Tag

N-His

Sequence

Thr 456-Asp 590

Field of Research

Signal Transduction; Neuroscience

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.