Recombinant Rat AIF-M1 protein (His tag)

Product Specifications

Background

Probable oxidoreductase that has a dual role in controlling cellular life and death, during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.

Abbreviation

AIF-M1

Synonyms

AIFM1; Apoptosis inducing factor; Apoptosis inducing factor 1; CMTX4; COWCK; COXPD6; Harlequin; PDCD 8; Programmed cell death 8; Striatal apoptosis inducing factor; mitochondrial

UniProt

Q9JM53

Accession Number

Q9JM53

Expression System

E.coli

Tag

N-His

Sequence

Arg 150-Val 299

Field of Research

Cell Biology; Epigenetics and Nuclear Signaling; Cancer; Metabolism; Neuroscience

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.