Recombinant Rat C4 protein (His tag)
Product Specifications
Background
Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes.
Abbreviation
C4
Synonyms
C4; C4a; Complement C4; Complement C4 beta chain
UniProt
P08649
Accession Number
P08649
Expression System
E.coli
Tag
N-His
Sequence
Lys 20–Ser 673
Field of Research
Immunology
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method.
Purity
> 95% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
71.8 kDa
Observed Molecular Weight
70 kDa
Species
Rat
Available Sizes
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