Recombinant Rat MMP-12 protein (His tag)
Product Specifications
Background
Matrix metalloproteinase-12 (MMP12) is a secreted protein.It contains 4 hemopexin repeats and belongs to the peptidase M10A family. MMP12 may be involved in tissue injury and remodeling and have significant elastolytic activity. It can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
Abbreviation
MMP-12
Synonyms
Macrophage metalloelastase; MME; MMP-12; Matrix metalloproteinase-12; Mme; Mmel; Mmp12
UniProt
Q63341
Accession Number
Q63341
Expression System
E.coli
Tag
N-His
Sequence
Ala 286-Phe 463
Field of Research
Cancer; Cardiovascular; Signal Transduction
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method.
Purity
> 95% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
19.5 kDa
Observed Molecular Weight
24 kDa
Species
Rat
Available Sizes
Documents
Frequently Asked Questions
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